How Is Trichomonas Vaginalis (TV) Diagnosed?

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Dr Sanina Mansoor holds MBBS degree from Yenepoya university,Mangalore.She has 8 years of experience working as a medical officer at various health centres and medical colleges.
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Updated on 26 February, 2025
- Article was updated as part of our commitment to diversity, equity, and inclusion.

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Trichomonas vaginalis (TV) is a parasitic protozoan that causes trichomoniasis, a common sexually transmitted infection (STI). Diagnosing TV involves several methods aimed at detecting the presence of the parasite in bodily fluids or tissues.
Primary Diagnostic Methods Used For Trichomonas Vaginalis
- Physical Examination: During a physical examination, healthcare providers may look for any visible signs of trichomoniasis. This can include observing vaginal discharge in women, which may appear frothy, yellow-green, or grayish with a strong odor. In men, physical signs may be less obvious but can include irritation or inflammation of the urethra.
- Microscopic Examination: Microscopic examination of samples is a common method for diagnosing TV. For women, a sample of vaginal fluid or discharge is collected using a swab and then examined under a microscope for the presence of Trichomonas vaginalis parasites. In men, a sample of urethral fluid or discharge can be similarly examined.
- Nucleic Acid Amplification Tests (NAATs): NAATs are highly sensitive and specific tests that detect the genetic material (DNA or RNA) of Trichomonas vaginalis. These tests can be performed on various types of samples, including vaginal or cervical swabs in women and urethral swabs or urine samples in men. NAATs are considered one of the most reliable methods for diagnosing TV due to their high accuracy.
- Cultures: Cultures involve growing Trichomonas vaginalis in a laboratory setting from a sample taken from the infected area. While cultures can be used to diagnose TV, they are less commonly used than NAATs due to their longer turnaround time and lower sensitivity compared to molecular tests.
- Point-of-Care Tests: Some rapid tests are available that provide quick results for diagnosing TV. These tests may use a lateral flow immunoassay or similar technology to detect specific antigens or antibodies associated with Trichomonas vaginalis. While point-of-care tests offer convenience, their sensitivity and specificity may vary, so confirmation with more definitive tests like NAATs is often recommended.
- Partner Screening: Since trichomoniasis is a sexually transmitted infection, healthcare providers may recommend screening and testing for Trichomonas vaginalis in sexual partners of individuals diagnosed with the infection. This helps prevent reinfection and the spread of the parasite.
It’s important to note that accurate diagnosis is crucial for effective treatment of Trichomonas vaginalis infection. If you suspect you have trichomoniasis or have been exposed to the infection, it’s essential to consult a healthcare professional for proper testing and management.
